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fixable viability dye (fvd) efluor506  (Thermo Fisher)


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    Structured Review

    Thermo Fisher fixable viability dye (fvd) efluor506
    Fixable Viability Dye (Fvd) Efluor506, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fixable+viability+dye+efluor506/fixable+viability+dye/pm40389394-192-15-22
    Average 90 stars, based on 1 article reviews
    fixable viability dye (fvd) efluor506 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Microbiota-produced immune regulatory bile acid metabolites control central nervous system autoimmunity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER QIAamp PowerFecal Pro DNA Kit QIAGEN Cat# 51804; Nextera DNA Flex Library Preparation Kit Illumina Cat# 20018705; FOXP3/Transcription Factor Staining Buffer Set ThermoFisher Cat# 00-5523-00; Cytofix/Cytoperm kit BD Cat# 555280; RNeasy Mini Kit QIAGEN Cat#74104; SuperScript III First-Strand Synthesis System Life Technologies Cat#18080051; SYBR Select Master Mix Life Technologies Cat# 4472908; Lamina Propria Dissociation Kit, mouse Miltenyi Biotec Cat# 130-097-410; Leukocytes Activation Cocktail with BD GolgiPlugTM BD Bioscience Cat# 550583; Mouse GM-CSF Miltenyi Biotec Cat# 130-095-746; Mouse IL-4 Miltenyi Biotec Cat# 130-097-757; Lipopolysaccharide Sigma-Aldrich Cat# L2880; Naive CD4+ T cell isolation kit, mouse Miltenyi Biotec Cat# 130-104-453; Deposited data Shotgun metagenomics, NCBI GEO repository This paper Accession ID GEO: GSE233771 UPLC/MS metabolomic data, Zenodo platform This paper https://doi.org/10.5281/zenodo.

    Article Title: Microbiota-produced immune regulatory bile acid metabolites control central nervous system autoimmunity.
    Article Snippet: The staining of nuclear transcription factors was performed using the FOXP3/Transcription Factor Staining Buffer Set (ThermoFisher).

    Flow Cytometry:

    Article Title: Selective depletion of CCR8+Treg cells enhances anti-tumor immunity of cytotoxic T cells in lung cancer via dendritic cells.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Single Cell:

    Article Title: Selective depletion of CCR8+Treg cells enhances anti-tumor immunity of cytotoxic T cells in lung cancer via dendritic cells.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Staining:

    Article Title: Selective depletion of CCR8+Treg cells enhances anti-tumor immunity of cytotoxic T cells in lung cancer via dendritic cells.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Microbiota-produced immune regulatory bile acid metabolites control central nervous system autoimmunity
    Article Snippet: .. Cells were first stained with Fixable Viability dye eFluor506 (ThermoFisher) diluted in PBS (1:500) for live/dead cell discrimination, and then incubated 20 min at 4°C in the dark with a combination of fluorochrome-conjugated surface antibodies in staining buffer. .. The staining of nuclear transcription factors was performed using the FOXP3/Transcription Factor Staining Buffer Set (ThermoFisher).

    Article Title: B cell subsets have different capacities for phagocytosis and subsequent presentation of antigen to cognate T cells
    Article Snippet: .. Dead cells were identified by staining with the Fixable Viability Dye eFluor506 (eBioscience) according to the manufacturer’s protocol. ..

    Article Title: Microbiota-produced immune regulatory bile acid metabolites control central nervous system autoimmunity.
    Article Snippet: Briefly, cells were suspended in PBS and dead cells were stained with Fixable Viability Dye eFluor506 (ThermoFisher). .. Briefly, cells were suspended in PBS and dead cells were stained with Fixable Viability Dye eFluor506 (ThermoFisher). .. Cells were then fixed and permeabilized by using the BD Cytofix/Cytoperm kit, and finally stained for intracellular cytokines.

    Incubation:

    Article Title: Microbiota-produced immune regulatory bile acid metabolites control central nervous system autoimmunity
    Article Snippet: .. Cells were first stained with Fixable Viability dye eFluor506 (ThermoFisher) diluted in PBS (1:500) for live/dead cell discrimination, and then incubated 20 min at 4°C in the dark with a combination of fluorochrome-conjugated surface antibodies in staining buffer. .. The staining of nuclear transcription factors was performed using the FOXP3/Transcription Factor Staining Buffer Set (ThermoFisher).

    Bioprocessing:

    Article Title: DDO-adjuvanted influenza A virus nucleoprotein mRNA vaccine induces robust humoral and cellular type 1 immune responses and protects mice from challenge
    Article Snippet: .. Fixable Viability Dye eFluor506 and monoclonal antibodies specific for mouse IFNγ (XMG1.2), Ly6c (HK1.4), CD3 (17A2), CD19 (eBio1D3), B220 (RA3-6B2), NK1.1 (PK136), CD11b (M1/70), and TNFα (MP6-XT22) were obtained from eBioscience. .. Monoclonal antibodies specific for mouse CD3 (17A2), CD4 (GK1.5), CD11a (H11578), XCR1 (ZET), PDCA1 (129 cl), CD11c (cN418), SIRPα (P84), CD64 (x54/7.1), CD44 (NIM-R8), CD62L (cMEL-14), CCR7 (4B12), CD103 (QA17A24) and MHC-II (M5/114.15.2) were obtained from BioLegend.



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